deproteinisation with acetonitrile (along with methanol or other reagents) is a useful and rapid technique in analysis of drugs or their metabolites in human serum. in this paper application of this simple technique in biopharmaceutical analysis using capillary electrophoresis (ce) is evaluated. some drugs with different ionic and protein binding properties were selected and dissolved in human ...

a capillary electrophoresis method was used for assay of some degradation products of carvedilol. the optimized parameters were as; running buffer 80 mm acetate dissolved in methanol/ethanol mixture (65:35 v/v), applied voltage of 19 kv, temperature is 20 ºc and the wavelength range of 200-350 nm. the results indicate that the proposed ce method could effectively separate carvedilol from its de...

capillary electrophoresis (ce) with indirect uv detection is an interesting analytical method for the analysis of drugs and pharmaceuticals. good and reproducible capillary quality is needed to develop robust methods and to facilitate method transfer in ce. it is widely accepted that preconditioning procedures are indispensable in capillary electrophoresis in order to achieve reproducibility of...

isolation of cell free fetal dna (cffdna) from maternal serum usually leads to very low concentrations of dna impeding further resolving through conventional methods of electrophoresis. although several protocols have been described for capillary electrophoresis (ce) of double stranded dna, they usually need using special polymers or coated capillaries which degrade over time. herein, we propos...

the phenomenological model of khossravi and connors (1992) has been adopted to calculate the electrophoretic mobility of drugs at different concentrations of solvents in a binary mixture. the accuracy and predictability of the model have been evaluated employing 14 experimental data sets by using average percentage mean deviation (apmd). the obtained apmd for correlative and predictive studies ...

carvedilol is administered as a racemic mixture of the r(+)- and s(-)-enantiomers, although it was demonstrated that the two enantiomers exhibit different pharmacological effects and stereoselective pharmacokinetics. the aim of this study was the evaluation of several native and derivatized cyclodextrines as chiral selectors for the separation of carvedilol enantiomers. stereoselective interact...

abstract         background and objective: normal hemoglobin (hb) is formed of a heme group and a protein group known as globin. globin is made of four polypeptide chains and in hemoglobinopathies, the structure of one of these four polypeptide chain becomes abnormal. cellulose acetate method is a common way to differentiate haemoglobinopathies. inability to identify the components of hb low co...

• Capillary electrophoresis is well adapted for amino acids analysis. associated to fluorescence results in nanomolar concentration detections. conductimetry able be used most applications. mass spectrometry a very useful tool metabolomic Enantio-separations of are easily obtained using capillary electrophoresis.

Background: Hemoglobinopathies are the commonest single gene disorder in human that affect hemoglobin production and function that occur when mutations alter the amino acid sequence of globin chains. The purpose of the present study was to evaluate the prevalence of hemoglobninopathies detected by capillary electrophoresis method in individuals referred to Masjed-Soleiman health centers by capi...